Journal: Bioresources and Bioprocessing

Article Title: Heterologous expression and functional characterization of recombinant arenin to assess its anticancer and wound-healing potential

doi: 10.1186/s40643-025-00986-2

Figure Lengend Snippet: Viability of human dermal fibroblasts (HDFa), breast adenocarcinoma cells (MCF-7), and colorectal adenocarcinoma cells (Caco-2) after 48 h exposure to arenin. Data are expressed as the percentage of MTS-formazan absorbance (490 nm) relative to untreated controls (mean ± SD, n = 3). Statistical analysis was performed with one-way ANOVA followed by Tukey’s multiple-comparison test; Different letters abcde indicate statistical differences analyzed using the Tukey test ( p < 0.05)

Article Snippet: Human breast adenocarcinoma (MCF-7), human colorectal adenocarcinoma (Caco-2), and human primary dermal fibroblasts (HDFa) cells were purchased from the American Type Culture Collection (ATCC®, Manassas, VA, USA).

Techniques: Comparison

Journal: Bioresources and Bioprocessing

Article Title: Heterologous expression and functional characterization of recombinant arenin to assess its anticancer and wound-healing potential

doi: 10.1186/s40643-025-00986-2

Figure Lengend Snippet: Effect of arenin on HDFa cell migration under high glucose (HG) and serum-deprived (FBS–) conditions. Scratch wound healing assays were performed in monolayers treated with arenin (31.25–1000 µg mL −1 ), Centella asiatica extract (CA, 10 µg mL −1 ), or left untreated (C–). A , C Time course of wound retraction (%) under FBS– and HG conditions at 24, 48, and 72 h. B , D Wound closure (%) after 72 h in FBS– and HG conditions. E Phase contrast micrographs at 0 and 72 h under FBS– (upper) and HG (lower) conditions show C–, CA, and arenin treatments. Dashed lines separate controls from arenin. Complete closure is observed in arenin- and CA-treated cultures, while sizeable gaps persist in C–. Bars/data points = mean ± SD ( n = 3). One-way ANOVA with Tukey’s post hoc test; groups sharing the same lowercase letter are not significantly different ( p > 0.05). Scale bars = 200 µm

Article Snippet: Human breast adenocarcinoma (MCF-7), human colorectal adenocarcinoma (Caco-2), and human primary dermal fibroblasts (HDFa) cells were purchased from the American Type Culture Collection (ATCC®, Manassas, VA, USA).

Techniques: Migration